Candidatures et postes

General Information:

An MSc position is open in the Tóth lab to conduct structure-function studies on a newly discovered cellular organelle (Anastasia et al. Cell Reports, 2021). The candidate will learn to use state-of-the-art imaging methodologies such as electron tomography to reconstruct the various compartments of the cell in 3D. In addition, they will learn how to design, test, and analyze quantitative electron microscopy data and present them to both scientific and external audiences. Through this research program, the candidate will learn to recognize, evaluate, and solve any complex problem using the scientific method. The student will be part of a highly dynamic and collaborative project.

Please, send your CV to ktoth@uottawa.ca

Desired qualifications:

Desired Qualifications:

• Knowledge and experience in fundamental methods in cell and molecular biology;
• Strong organizational skills, attention to detail and effective problem-solving abilities;
• Efficient time management skills;
• Ability to work independently and as part of a team;
• Knowledge and experience in safe laboratory practices;
• Experience with management of mouse colonies;
• Bilingualism along with good interpersonal and communication skills an asset.

Responsibilities and tasks:

*The person hired for this position will be responsible for the following routine tasks:
 

General lab maintenance:

• Effective organization of glassware cleaning and sterilization.
• to keep the lab organized and clean.
• to verify and maintain CO₂ tanks and liquid N₂ cryopreservation tanks.
• to ensure all basic laboratory equipment is properly calibrated and functional.

General operating tasks:

• to keep track of inventory for basic lab supplies.
• to prepare order requisitions when needed.
• to obtain orders as they arrive at the Faculty or in local biobars.
• to keep a record of purchase orders and overall expenditures.

Mouse colonies:

• will be responsible for genotyping and record keeping of common mouse lines used on a regular basis.
• establish matings to generate and maintain appropriate mouse lines
• assist with analysis of mouse embryo or offspring.

Routine laboratory work:

• molecular biology, including cloning, plasmid preps, RT-PCR, qPCR, chromatin immunoprecipitation, etc.
• basic protein biochemistry, including immunoprecipitation, Western blotting.
• ability to learn new techniques.

Salary:

Will be based on experience and for 20 hours/week.

To apply:

Candidates should submit their CV and the names and contact information for two references to David Lohnes at dlohnes@uottawa.ca

Description:

The focus of the project is on the large-scale analysis of multi-omics datasets from human gut microbiome in the context of pediatric inflammatory bowel diseases. The goal of the project is to integrate the results of metagenomics, metatranscriptomics and metaproteomics datasets in a machine learning framework to identify predictive signatures of treatment responses in inflammatory bowel disease patients and classify them accordingly.

This research will take place at the Faculty of Medicine of the University of Ottawa. Ottawa is considered to be one of the best livable cities on the globe.

Basic Requirements:

Basic Requirements (Education/Experience):

The ideal candidate should:

• Have a Ph.D. in Bioinformatics, Computational Biology, Machine Learning, Biostatistics, Genomics, or a related field
• Have experience with omics data analysis from next-generation sequencing technologies
• Have knowledge and experience with large-scale biological data analysis
• Have experience in analyzing clinical datasets
• Have experience in the computational analysis of large biological datasets such as ENCODE and TCGA.
• Be experienced with machine learning techniques applied to large biological datasets
• Have proficiency in a programming language such as Java, C, C++, Python, R or Matlab.
• Be comfortable in a Linux environment.
• Be a team player
• Have the ability to work independently
• Have strong written and verbal communication skills and a good ability to communicate with colleagues in biological sciences
• Have a strong publication record.Additional assets considered for this position:
• Have experience in proteomics data analysis
• Have a working knowledge of annotation databases such as Gene Ontology, GeneCards, Ensembl, and Reactome
• Have experience working in a collaborative and multidisciplinary team environment

Start date and Salary:

Start Date: As soon as possible

Salary: $50,000 CAD/year with benefits

To apply:

Please submit a complete CV, a cover letter and the names and contact information of three references to Dr. Mathieu Lavallée-Adam at this address: mathieu.lavallee@uottawa.ca

General Information:

Postdoctoral positions are available in the laboratory of Dr. Xiaohui Zha at Ottawa Hospital Research Institute/University of Ottawa*, Canada (http://www.ohri.ca/profile/Zha).

Project Description:

The project is to study the mechanisms by which cholesterol regulates macrophage inflammation, a key event in atherosclerosis. Cholesterol, a major component of the plasma membrane in mammalian cells, predominantly regulates membrane physical properties. Most likely through its modulation of cellular membranes, cholesterol impacts numerous signal transduction pathways and cellular processes. Particularly, cholesterol levels in macrophages determine the nature of the inflammation response, for example, pro-inflammatory response (M1), anti-inflammatory (M2) or anything in between. The mechanism is largely unknown. In recent years, it is increasingly apparent that macrophages can respond to environmental cues to establish an epigenetically “poised” state. This allows macrophages to rapidly launch a large-scale gene expression program upon stimulation (i.e. LPS). The project will use biochemistry and genomic techniques, such as RNA-seq and ChIP-seq, to understand how cholesterol establishes epigenetically “poised” states in the genome of the macrophages to regulate gene expression for inflammatory responses.

Basic Requirements:

Candidates should be a fresh PhD (less than two years since graduation) in physical science or biological science with good publication record. Knowledge/experience in genomic system biology is a plus, but not necessary. If interested, please send curriculum vitae and brief statement of research interest to xzha@ohri.ca .

Contact info:

Xiaohui Zha, Senior Scientist
Chronic Disease Program
Ottawa Hospital Research Institute
501 Smyth Road, Ottawa, ON K1H 8L6
 

*The Ottawa Hospital Research Institute is the research arm of The Ottawa Hospital and an affiliated institute of the University of Ottawa. With more than 1,700 scientists, clinician investigators, trainees and staff, and total revenues of more than $100 million, it is one of the foremost Canadian hospital-based research institutes. Researcher are investigating virtually every major disease and condition, with a focus on answering important health questions and translating our findings into benefits for patients and society.