Transgenic Research Technician
Contact/Scientist: Dr. David Lohnes
Chair | Professor, Department of Cellular and Molecular Medicine
Director, Transgenic Core
Description of the position:
The principal mandate of the University of Ottawa Transgenic Core facility is to provide genetically modified mouse models to the research community (ES cell-based, transgenic, CRISPR/Cas9); to provide cryogenic archiving of existing mouse lines; and to provide embryo transfer of contaminated mouse lines. These services will be initiated by a Research Technician who will report to the Director of the core (currently Dr. David Lohnes of CMM). This technician will be responsible for the day-to-day operation of the core and development of new technologies.
- In consultation with the ACVS at RGN, to develop and implement and maintain Standard Operating Procedures and protocols for the derivation of transgenic and knockout models as well as cryopreservation and embryo transfer.
- To assist in the development and implementation of a web-based portal for dissemination of information regarding the Transgenic core, and for interacting with clients.
- In collaboration with the Director of the core, to consult with clients as to the design and preparation of transgenic constructs.
- Responsible for all aspects of derivation of transgenic mice, including quality control and purification of transgenes, microinjection of transgenic DNA constructs and ES cells, assistance in the genotyping of transgenic offspring, establishing mouse colonies for the generation of fertilized eggs, recipient pseudopregnant females and other aspects related to routine transgenic technology.
- Responsible for ordering animals, supplies and equipment for the generation of transgenic offspring and cryogenesis.
- Responsible for cryogenic archiving of mouse embryos.
- Responsible for accurate record keeping of all orders, generation of transgenic offspring and cryogenic archival records.
- Assessment and development of new technologies related to mouse modeling
Highly-motivated candidates are encouraged to email a cover letter, updated CV and a page listing the names and contacts of 3 referees to firstname.lastname@example.org.
Postdoctoral Research Fellow
Contact/Scientist: Dr. Jing Wang
Professor, Department of Cellular and Molecular Medicine
Program: Regenerative Medicine
Description of the position:
Our research focuses on delineating molecular mechanisms that regulate proliferation and differentiation of neural stem cells, with the ultimate goal of the development of stem cell- based therapy to promote neural repair in the context of various neurological disorders. The ongoing project in the lab is targeting the aPKC-CBP pathway to promote neural stem cell reprogramming differentiation in the context of neurodegenerative diseases including stroke, multiple sclerosis and Alzheimer’s disease. A variety of molecular, cellular, and behavioral techniques are used in the laboratory, including transgenic mouse models, neurosphere culture, reprogramming cell culture and stereotaxic surgery, etc.
- Experience in Molecular Biology (cloning, mutagenesis, PCR), Cell Biology (cell line or primary cell culture, immunohistochemistry and immunocytochemistry) and in vivo animal model study.
- Excellence in research with strong publication records
- Excellent written and oral communication skills
- Experience with Human iPSC-derived cell culture is an asset
Until the position is filled.
Individuals who are interested in the position should submit curriculum vitae, a copy of official transcripts, statement of research interest and names of three references to:
Dr. Jing Wang, Ph.D.
Scientist Sprott Centre for Stem Cell Research
Ottawa Hospital Research Institute
Assistant Professor Department of Cellular & Molecular Medicine
University of Ottawa
Ottawa Hospital, 501 Smyth Rd, Box 511
Ottawa, ON K1H 8L6 CANADA
The Ottawa Hospital Research Institute (OHRI) is an Affiliated Research Institute of the University of Ottawa and the research arm of The Ottawa Hospital. The OHRI has grown rapidly to become one of the largest and most important hospital-based research institutes in Canada.
We thank all applicants for their interest. However, only those invited for an interview will be contacted.
The Faculty of Medicine at the University of Ottawa, in collaboration with the Regenerative Medicine Program of the Ottawa Hospital Research Institute, invites applications for a tenure-track position in regenerative medicine. Click here for more information.