Maxwell Hincke

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Maxwell Hincke
Cross-Appointed Member, Professor

Room: RGN 2235
Office: 613-562-5800 ext. 8193
Work E-mail: mhincke@uottawa.ca

Dr. Max Hincke

Biography

  • Evolution of innate immunity at biomineralized barriers.
    A productive theme of my research program has been directed at understanding the innate immunity of the avian egg, where the eggshell provides a barrier function and acts in concert with antimicrobial proteins to maintain the pathogen-free status of the egg. These features are essential for food safety of the unfertilized egg, are key elements of avian reproduction and reveal the egg contents as a bio-inspiration for identification of novel antimicrobials. My studies have provided important insight into how molecular regulation of calcitic biomineralization is exerted during eggshell calcification, and identified a variety of antimicrobial strategies that can be exploited by reverse-engineering for application to human health and therapeutics.

    Selected Publications (since 2010).
    • Athanasiadou, D., Jiang, W., Goldbaum, D., Basu, K., Pacella, M.S., Böhm, C.F., Chromik, R.R., Hincke, M.T., Rodríguez-Navarro, A.B., Vali, H., Wolf, S.E., Gray, J.J., Bui, K.H. and McKee, M.D. (2018) Nanostructure, osteopontin and mechanical properties of avian calcitic eggshell. Science Advances (in press).
    • Jodoin, J. and Hincke, M. T. (2018) Histone H5 is a potent Antimicrobial Agent and a template for novel Antimicrobial Peptides. Scientific Reports, 8:2411. DOI: 10.1038/s41598-018-20912-1.
    • Kulshreshtha, G., Rodriguez-Navarro, A., Sanchez-Rodriguez, E., Diep, T. and Hincke, M.T. (2018) Cuticle and pore plug properties in the table egg. Poultry Science doi: 10.3382/ps/pex409.
    • Rose-Martel, M., Kulshreshtha, G., Berhane, N.A., Jodoin, J. and Hincke, M.T. (2017) Histones from Avian Erythrocytes Exhibit Antibiofilm activity against methicillin-sensitive and methicillin-resistant Staphylococcus aureus.  Scientific Reports, 7: 45980. doi: 10.1038/srep45980.
    • Ahmed, T.A.E., Suso, H.P. and Hincke, M.T. (2017) In-depth Comparative Analysis of the Chicken Eggshell Membrane Proteome. J. Proteomics, 155: 49-62.
    • Cordeiro, C.M.M. and Hincke, M.T. (2016) Quantitative proteomics analysis of eggshell membrane proteins during chick embryonic development. J. Proteomics 130: 11-25.
    • Rose-Martel, M., Smiley, S. and Hincke, M.T. (2015) Novel identification of matrix proteins involved in calcitic biomineralization. J. Proteomics. 116: 81-96.
    • Alejandro B. Rodríguez-Navarro, Pauline Marie, Yves Nys, Maxwell T. Hincke, Joel Gautron. (2015) Amorphous calcium carbonate controls avian eggshell mineralization: a new paradigm for understanding rapid eggshell calcification. J. Structural Biology. 190(3): 291-303.
    • Du J, Hincke MT, Rose-Martel M, Hennequet-Antier C, Brionne A, Cogburn LA, Nys Y, Gautron J. (2015)  Identifying specific proteins involved in eggshell membrane formation using gene expression analysis and bioinformatics. BMC Genomics. 16: 792.
    • Zhang, Q., Liu, L., Zhu, F., Ning, Z.H., Hincke, M.T., Yang, N. and Hou, Z.H. (2014) Integrating de novo Transcriptome Assembly and Cloning to Obtain Chicken Ovocleidin-17 Full-Length cDNA. PLoS ONE. 9(3): e93452.
    • Rose-Martel, M. and Hincke, M.T (2014) Antimicrobial histones from chicken erythrocytes bind bacterial cell wall lipopolysaccharides and lipoteichoic acids. International Journal of Antimicrobial Agents. 44: 470-472
    • Kovacs-Nolan, J., Cordeiro, C., Young, D., Mine, Y., and Hincke, M.T. (2014) Ovocalyxin-36 is an effector protein modulating the production of proinflammatory mediators.  Veterinary Immunology and Immunopathology. 160: 1-11.
    • Hincke, M.T. (2013) Understanding control of calcitic biomineralization Proteomics to the rescue. Proteomics. 13(23-24): 3369-70.
    • Cordeiro, C.M.M., Esmaili, H., Ansah, G. and Hincke, M.T. (2013) Ovocalyxin-36 is a pattern recognition protein in chicken eggshell membranes. PLOS One. 8(12): e84112.
    • Hincke MT, Nys Y, Gautron J, Mann K, Rodriguez-Navarro AB and McKee MD (2012) The eggshell: structure, composition and mineralization. Frontiers in Biosciences special edition on Biomineralization. 17: 1266-1280.
    • Rose-Martel, M., Du, J. and Hincke, M.T. (2012) Proteomic analysis provides new insight into the chicken eggshell cuticle. J. Proteomics 75: 2697-2706.
    • Gautron J., Rehault-Godbert S., Pascal G., Nys Y. and Hincke M.T. (2011) Ovocalyxin- 36 and other LBP/BPI/Plunc like proteins as molecular actors of the mechanisms of the avian egg natural defences. Biochem. Soc. Transactions. 39: 971-976.
    • Loit, E., Hincke, M. T., Altosaar, I. (2010) Synthetic antimicrobial peptide L8, MHLHKTSRVTLYLL, has membrane permeabilization and bacterial aggregation activity. International Journal of Antimicrobial Agents, 35(4):410-1.
    • Hincke MT, Nys Y and Gautron J (2010) The Role of matrix proteins in eggshell formation. Journal of Poultry Science, 47 (3): 208-219.
    • Réhault-Godbert, S., Baron F., Mignon-Grasteau, S., Labas, V., Michel Gautier, Hincke, M.T. and Nys, Y. (2010) Effect of temperature and time of storage on protein stability and anti-Salmonella activity of egg white. Journal of Food Protection, 73(9):1604-12.
    • Wellman-Labadie, O., Lemaire, S., Mann, K., Picman, J. and Hincke, M.T. (2010) Antimicrobial Activity of Lipophilic Avian Eggshell Surface Extracts. J. Agricultural and Food Chemistry, 58(18):10156-61.
    • Nys Y., Hincke M., Hernández-Hernández A., Rodríguez-Navarro A.B., Gómez-Morales J., Jonchère V., García-Ruiz J.M. and Gautron J. (2010) Structure, propriétés et minéralisation de la coquille de l’oeuf. Productions Animales, 23 (2): 143-154.
    • Cordeiro, C. and Hincke, M.T. (2010) Eggshell: Shell and Membranes Applications (review). Recent Patents on Food, Nutrition& Agriculture, 3(1): 1-8.
  • Regenerative Medicine: Tissue engineering for articular cartilage.
    Canadian and other western societies face a demographic imperative. Canada's health care system is confronted by an aging population which is living longer with greater expectations of mobility and quality of life. Age-related wear and tear of cartilage (osteoarthritis) and traumatic cartilage damage are the leading cause of disability in Canada. Health problems associated with deterioration of articular cartilage affect over one million patients per year in North America and are the major cause of orthopedic interventions. In 2000/01, arthritis and related diseases affected over 1.6 million Ontarians aged 15 yr or older; these numbers will double by 2031. In 2001/02, 18% of total hip replacement (THR) and 10% of total knee replacement (TKR) surgeries in Ontario were revisions - necessary due to failure of prostheses from previous surgery. Given cumulative and increasing numbers of THR and TKR surgeries, revision numbers will continue to rise. A more permanent regenerative medicine solution for damaged cartilage will improve patient well-being and quality of life, and lead to reduced health care costs. The goal of this research program is to develop implantable articular cartilage using bioengineered scaffolds that function as regeneration templates. Semi-synthetic scaffolds will serve as scaffolds for chondrogenic cell precursors and stem cells to form the normal structure of cartilage for integration into the surrounding healthy tissue. Approaches such as this will lead to reduced health care costs to support a sustainable Canadian health care system.

    Selected Publications (since 2010).
    • Hamza, D.A.M., Ahmed, T.A. and Hincke, M.T. (2017) Corneas: Tissue Engineering. In: Concise Encyclopedia of Biomedical Polymers and Polymeric Biomaterials. Taylor & Francis. Pp. 370-394.
    • Ahmed, T.A.E. & Hincke, M.T. (2014) Mesenchymal stem cell based tissue engineering strategies for repair of articular cartilage. Histology & Histopathology. 29: 669-689.
    • Ahmed, T.A.E. and Hincke, M.T. (2012) Fibrin for encapsulation of human mesenchymal stem cells for chondrogenic differentiation. In: Stem Cells and Cancer Stem Cells, Volume 10. (Ed. M. A. Hayat) Springer Publishing. Pp 59-69.
    • Ahmed, T., Griffith, C.M., Giulivi, A. and Hincke, M.T. (2011) Fibrin glues in combination with mesenchymal stem cells to develop a tissue-engineered cartilage substitute. Tissue Engineering Part A, 17(3-4): 323-35.
    • Ahmed, T., Hincke, M.T. (2010) Strategies for Articular Cartilage Lesion Repair and Functional Restoration. Tissue Engineering; Part B, 16(3):305-29.
  • Medical Education: Human Anatomy and Anatomical Sciences Education.Anatomy is one of the most important basic science disciplines to teach well in a medical school. The anatomical sciences are a gateway to the clinical world for medical and health science students. Through its study they come to understand the rich tapestry of structures to be encountered in every patient, while appreciating at the same time the variability that each individual possesses. The study of Anatomy introduces students to the specialized vocabulary of medicine, and helps them to begin to deal with issues of death that are inevitable in clinical practice.

    Selected Publications (since 2010).
    • Ashdown, L.C., Lewis, E., Hincke, M.T. and Jalali, A. (2013) Learning Anatomy: Can dissection and peer-mediated teaching offer added benefits over prosection alone? ISRN Anatomy, Volume 2013, Article ID 873825, 4 pages. http://dx.doi.org/10.5402/2013/873825.
    • Weber, J., Hincke, M., Patasi, B., Jalali, A. and Wiper-Bergeron, N. (2012) The Virtual Anatomy Lab: an eDemonstrator pedagogical agent can simulate student-faculty interaction and promote student engagement. Medical Education Development, 2 (1): 10.4081/med.2012.e5.
    • Jalali, A., Trottier, D., Tremblay, M. and Hincke, M. (2011) Administering a Gross Anatomy Exam Using Mobile Technology: How one medical school made the switch from paper to paperless. eLearn Magazine 2: doi>10.1145/1943208.1943209.
    • Jalali, A., Leddy, J., Gauthier, M., Sun, R., Hincke, M. and Carnegie, J. (2011) Use of Podcasting as an Innovative Asynchronous E-Learning Tool for Students. US-China Education Review. US-China Education Review A 11: 741-748.

Fields of Interest

  • Molecular dynamics of Connective tissue
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