DaSilva, Jean N.

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DaSilva, Jean N.
Professor

Tel: 613) 798-5555 ext. 19704
Fax: (613) 761-5406
Work E-mail: jdasilva@ottawaheart.ca

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Biography

Positron emission tomography (PET) is an imaging technique using radioactive physiological tracers as molecular probes that can be used to study non-invasively cellular pathways that regulate cardiovascular function in normal and disease states in living organisms with high resolution. Our group works on various projects using radioligands for measuring blood flow, metabolism and neurohormonal pathways in cardiovascular disorders, and diabetes. Current field of interests are focused on imaging: A) glucose, fatty acid and oxidative myocardial metabolism; B) the noradrenergic signaling pathway at the transporter, receptors, and cAMP-mediated signal transduction; C) the renin-angiotensin system at the AT1 receptor level in heart, brain and kidney; D) stem cell labeling. The pharmacological binding profile of novel tracers will be evaluated in vitro and in vivo to assess their potential as quantitative markers in animal models of disease states. Our experimental approaches include: 1) radiosyntheses; 2) in vitro binding and autoradiographic studies; 3) biodistribution studies; 4) in vivo binding specificity and selectivity; 5) pharmacological modulation studies in animal models; 6) metabolite and dosimetry studies; 7) large animal PET studies; 8) pharmacokinetic modeling; 9) effect of therapeutic agents on targeted site; 10) PET scans in humans. Development and evaluation of these probes for imaging altered systems provides insights into understanding cardiovascular pathophysiology, help direct and guide therapy, and evaluate progression of disease/treatment using PET. Our research is enhanced by collaborations with various Investigators at the University of Ottawa.

Selected Publications

  • Thackeray, J., Beanlands, R.S., and DaSilva J.N. (2007) Presence of Specific [ 11C]meta-Hydroxyephedrine Retention in Heart, Lung, Pancreas, and Brown Adipose Tissue . J. Nucl Med. 48:1733-1740.
  • Ruel M., Beanlands R.S., Lortie M., Chan V., deKemp R.A., Suuronen E.J., Rubens F.D., DaSilva J.N., Sellke F.W., Stewart D.J., Mesana T.G. (2008) Concomitant Treatment with Oral L-Arginine Improves the Efficacy of Surgical Angiogenesis in Patients with Severe Diffuse Coronary Artery Disease: The Endothelial Modulation in Angiogenic Therapy (EMAT) Randomized Controlled Trial. J Thorac Cardiovasc Surg 135(4):762-770.
  • Kenk M., Greene M., Lortie M., deKemp R.A., Beanlands R.S., DaSilva J.N. (2008) Use of a column-switching high-performance liquid chromatography method to assess the presence of specific binding of (R)- and (S)-[ 11C]rolipram and their labeled metabolites to the phosphodiesterase-4 enzyme in rat plasma and tissues. Nucl Med Biol 35:515-521.
  • Burwash I.G., Lortie M., Pibarot P., de Kemp R.A., Graf S., Mundigler G., Khorsand A., Blais C., Baumgartner H., Dumesnil J.G., Hacicha Z., DaSilva J.N., Beanlands R.S. Myocardial Blood Flow in Patients with Low Flow, Low Gradient Aortic Stenosis Differences Between True and Pseudo-Severe Aortic Stenosis - Results from the Multicenter TOPAS (Truly or Pseudo-Severe Aortic Stenosis) Study. Heart (In press).
  • Ukkonen H., Burwash I., deKemp R.A., Haddad H., Dafoe W., Yoshinaga Y., Davies R.A., DaSilva J., Beanlands R.S. Is ventilatory efficiency (VE/ VCO2 slope) associated with right ventricular oxidative metabolism in patients with congestive heart failure? Eur J Heart Failure (In press).
  • Zhang Y., Ruel M., Beanlands R.S.B., deKemp R.A.. Suuronen E., and DaSilva, J.N. Tracking stem cell therapy: applications of positron emission tomography. Curr. Pharm. Des. (In press).
  • Zhang Y., Thorn S., DaSilva J.N., Lamoureux M., deKemp R.A., Beanlands R.S., Ruel M. and Suuronen E., and DaSilva, J.N. Collagen-based matrices improve the delivery of transplanted circulating progenitor cells: development and demonstrations by ex vivo radionuclide cell labeling and in vivo tracking with positron emission tomography. Circulation Cardiovascular Imaging (In press).
  • Greene M., Thackeray J., Kenk M., Thorn S., Harper M.E., Beanlands R.S., and DaSilva J.N. Reduced in vivo phosphodiesterase-4 response to noradrenergic challenge in diet-induced obese rat brain, heart, and skeletal muscle. Can J Physiol Pharmacol. (In press)

Fields of Interest

  • Imaging blood flow
  • metabolism and signal transduction pathways with positron emission tomography
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