Zeiss LSM510 AxioImager M1

LSM 510 M1
Microsocpe Specifications

Stand model

Zeiss AxioImager M2 [upright]

Microscope controls

Fully automated objective nosepiece, reflector turret, condenser and light path control

Manual stage control (x,y)

Motorized focus control (z)

Software

Zen2009

Capabilities

  • DIC with confocal acquisition
  • Confocal optical sectioning
  • Live cell imaging
  • Multi-channel imaging
  • Timelapse
  • Z-stack
  • Tile scanning
  • FRAP, FLIP, FRET, photo-conversion

Objectives

  • 10x, 0.3 NA, Air, EC Plan-Neofluar (DIC)
  • 20x, 0.8 NA, Air, Plan-Apo (DIC II)
  • 40x, 1.3 NA, Oil, EC Plan-Neofluar (DIC III)
  • 40x, 0.8 NA, Water, Achroplan (DIC III)
  • 63x, 1.4 NA, Oil, Plan-Apo (DIC III)
  • 63x, 1.0 NA, Water, Plan-Apo (DIC III)
Light sources
  • X-Cite 120 Mercury lamp
  • Halogen lamp
  • 6 laser lines: 458nm, 477nm, 488nm, 514nm, 543nm, 633nm

Confocal Filters

  • Main beam splitter between Ch2 and Ch3: 490nm, 515nm, 545nm

Ch2 Filters

  • LP 420nm
  • BP 420nm-480nm
  • LP 505nm
  • BP 505nm-530nm
  • BP 505nm-550nm

Ch3 Filters

  • BP 505nm-530nm
  • BP 505nm-550nm
  • BP 560nm-615nm
  • LP 615nm
  • LP 655nm
Epifluorescence Filtersets
  • Blue Ex G365nm, FT 395nm, Em 420nm LP
  • Cyan Ex 436/20nm, FT 455nm, Em 480/40nm
  • Green Ex 470/40nm, FT 495nm, Em 525/50nm
  • Yellow Ex 500/20nm, FT 515nm, Em 535/30nm
  • Red Ex 560/40nm, FT 585nm, Em 630/75nm

Detectors

3 PMTs

Location

RGN 4502

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