Zeiss LSM510 AxioImager M1

Zeiss AxioImager M2 [upright]

Fully automated objective nosepiece, reflector turret, condenser and light path control

Manual stage control (x,y)

Motorized focus control (z)

Zen2009

• DIC with confocal acquisition
• Confocal optical sectioning
• Live cell imaging
• Multi-channel imaging
• Timelapse
• Z-stack
• Tile scanning
• FRAP, FLIP, FRET, photo-conversion

• 10x, 0.3 NA, Air, EC Plan-Neofluar (DIC)
• 20x, 0.8 NA, Air, Plan-Apo (DIC II)
• 40x, 1.3 NA, Oil, EC Plan-Neofluar (DIC III)
• 40x, 0.8 NA, Water, Achroplan (DIC III)
• 63x, 1.4 NA, Oil, Plan-Apo (DIC III)
• 63x, 1.0 NA, Water, Plan-Apo (DIC III)

• X-Cite 120 Mercury lamp
• Halogen lamp
• 6 laser lines: 458nm, 477nm, 488nm, 514nm, 543nm, 633nm

• Main beam splitter between Ch2 and Ch3: 490nm, 515nm, 545nm

Ch2 Filters

• LP 420nm
• BP 420nm-480nm
• LP 505nm
• BP 505nm-530nm
• BP 505nm-550nm

Ch3 Filters

• BP 505nm-530nm
• BP 505nm-550nm
• BP 560nm-615nm
• LP 615nm
• LP 655nm

• Blue Ex G365nm, FT 395nm, Em 420nm LP
• Cyan Ex 436/20nm, FT 455nm, Em 480/40nm
• Green Ex 470/40nm, FT 495nm, Em 525/50nm
• Yellow Ex 500/20nm, FT 515nm, Em 535/30nm
• Red Ex 560/40nm, FT 585nm, Em 630/75nm

3 PMTs

RGN 4502