Zeiss LSM510 AxioImager M1 [RGN 4502]

LSM 510 M1

Stand model

Zeiss AxioImager M2 [upright]

Microscope controls

Fully automated objective nosepiece, reflector turret, condenser and light path control

Fully motorized stage control (x, y) and focus control (z)

Software

Zen2009

Capabilities

DIC with confocal acquisition

Confocal optical sectioning

Live cell imaging

Multi-channel imaging

Timelapse

Z-stack

Tile scanning

FRAP, FLIP, FRET, photo-conversion

Objectives

10x, 0.3 NA, Air, EC Plan-Neofluar (DIC)

20x, 0.8 NA, Air, Plan-Apo (DIC II)

40x, 1.3 NA, Oil, EC Plan-Neofluar (DIC III)

40x, 0.8 NA, Water, Achroplan (DIC III)

63x, 1.4 NA, Oil, Plan-Apo (DIC III)

63x, 1.0 NA, Water, Plan-Apo (DIC III)

Light sources

X-Cite 120 Mercury lamp

Halogen lamp

6 laser lines: 458nm, 477nm, 488nm, 514nm, 543nm, 633nm

Filters

Confocal:

Main beam splitter between Ch2 and Ch3: 490nm, 515nm, 545nm

Ch2 filters

Ch3 filters

LP 420nm

BP 505nm-530nm

BP 420nm-480nm

BP 505nm-550nm

LP 505nm

BP 560nm-615nm

BP 505nm-530nm

LP 615nm

BP 505nm-550nm

LP 655nm

Epifluorescent filter sets:
Blue
Cyan
Green

Ex

G365

Ex

436/20

Ex

470/40

FT

395

FT

455

FT

495

Em

420 LP

Em

480/40

Em

525/50

Yellow
Red

 

Ex

500/20

Ex

560/40

 

FT

585

FT

858

 

Em

535/30

Em

630/75

 

 

Detectors

Confocal: 3 PMTs

Epifluorescence: AxioCam HRm CCD (monochrome)

Location

RGN 4502

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