Quorum Spinning Disk [RGN 3164B]

Quorum inverted spinning disk confocal microscope platform in University of Ottawa CBIA Core facility

Stand model

Leica DMI6000B [inverted]

Microscope controls

Fully automated objective nosepiece, reflector turret and light path control

Fully motorized stage (x, y) and focus control (z)

Manual interlock, camera selection

Environmental chamber control (temperature, CO2 and humidity)

Software

Metamorph

Capabilities

Brightfield, DIC and epifluorescence

Live cell imaging

Confocal optical sectioning

Multi-channel imaging

Timelapse

Z-stack

Tile scanning

FRAP, FLIP, photo-conversion (405nm laser only)

3D d-STORM

Objectives

20x, 0.7 NA, Air, HC Plan APO (DIC)

40x, 1.3 NA, Oil, PL APO (DIC)

63x, 1.4 NA, Oil, PL APO CS2 (DIC)

Light sources

X-Cite 120 Mercury light source

Halogen

4 laser lines: 405nm, 490nm, 561nm, 639nm

Filters

Confocal

Epifluorescence

Blue

 

Em

460/50

 

Green

Green

 

Em

525/36

Em

525/50

 

Red

Red

Red

Em

605/52

Em

620/60

Em

632/60

Far Red

 

Em

700/75

 

Blue

Ex

360/20

FT

400

Em

425

Green

Ex

470/40

FT

510

Em

515 LP

Red

Ex

538/44

FT

580

Em

590 LP

 

Detectors

EM-CCD camera: Hamamatsu EM-CCD ImagEM (512x512)

CCD camera: Hamamatsu ORCA-R2 (1344x1024)

Location

RGN 3164B

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