Zeiss LSM880 AxioObserver Z1 with AiryScan FAST

Zeiss LSM880 AxioObserver Z1 inverted confocal microscope with AiryScan FAST in University of Ottawa CBIA Core facility.
Microscope Specifications

Stand model

Zeiss AxioObserverZ1 mot [inverted]

Microscope controls

  • Fully automated objective nosepiece, reflector turret and light path control
  • Fully motorized stage (x, y) and focus control (z)
  • Environmental chamber control (temperature, CO2 and humidity), on stage environmental control (temperature, CO2 and humidity).

Software

ZenBlack 2.3

Capabilities

  • Confocal optical sectioning
  • Live cell imaging
  • Multi-channel imaging
  • Timelapse
  • Z-stack
  • Tile scanning
  • FRAP, FLIP, photo-conversion
  • Definite Focus
  • Spectral Imaging

Objectives

  • 20x, 0.8 NA, Air, Plan-Apo.
  • 40x, 1.3 NA, Oil, Plan-Apo
  • 40x, 1.2 NA, Water Corr. FCS
  • 63x, 1.4 NA, Oil, Plan-Apo

Light sources

  • X-Cite 110 LED
  • Transmitted Halogen
  • 6 laser lines: 405nm, 458nm, 488nm, 514nm, 561nm, 639nm

Filters

Confocal Detection

  • Adjustable band pass detection in 3 channels.

ChA (AiryScan) dual filters:

  • BP420-480 + BP495-550
  • BP420-480 + BP495-620
  • BP420-480 + LP650
  • BP465-505 + LP525
  • BP495-550 + LP570
  • BP570-620 + LP645

Detectors

  • Ch1- MA PMT
  • Ch 2-Cooled GaAsP (high sensitivity PMT)
  • Ch3- Cooled MA PMT
  • Ch1, Ch2 and Ch3 can be used simultaneously as spectral detectors (3nm increments)
  • ChT- T-PMT (transmitted light detector)
  • ChA- Airyscan (Cannot be combine with other detectors)

 

Location

RGN 3140

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