Zeiss LSM880 AxioObserver Z1 with AiryScan FAST [RGN 3140]

Zeiss LSM880 AxioObserver Z1 inverted confocal microscope with AiryScan FAST in University of Ottawa CBIA Core facility.

Stand model

Zeiss AxioObserverZ1 mot [inverted]

Microscope controls

Fully automated objective nosepiece, reflector turret and light path control

Fully motorized stage (x, y) and focus control (z)

Environmental chamber control (temperature, CO2 and humidity), on stage environmental control (temperature, CO2 and humidity).

Software

ZenBlack 2.3

Capabilities

Confocal optical sectioning

Live cell imaging

Multi-channel imaging

Timelapse

Z-stack

Tile scanning

FRAP, FLIP, photo-conversion

Definite Focus

Spectral Imaging

Objectives

20x, 0.8 NA, Air, Plan-Apo

40x, 1.3 NA, Oil, Plan-Apo

40x, 1.2 NA, Water Corr. FCS

63x, 1.4 NA, Oil, Plan-Apo

Light sources

X-Cite 110 LED

Transmitted Halogen

6 laser lines: 405nm, 458nm, 488nm, 514nm, 561nm, 639nm

Filters

Adjustable band pass detection in 3 channels. 

ChA (AiryScan) dual filters:

BP420-480 + BP495-550

BP420-480 + BP495-620

BP420-480 + LP650

BP465-505 + LP525

BP495-550 + LP570

BP570-620 + LP645

Detectors

Ch1- MA PMT

Ch 2-Cooled GaAsP (high sensitivity PMT)

Ch3- Cooled MA PMT

Ch1, Ch2 and Ch3 can be used simultaneously as spectral detectors (3nm increments)

 

ChA- Airyscan

ChT- T-PMT (transmitted light detector)

Location

RGN 3140

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