Zeiss LSM800 AxioObserver Z1 [RGN 3140]

Zeiss LSM800 AxioObserver Z1 inverted confocal microscope platform in University of Ottawa CBIA Core facility.

Stand model

Zeiss AxioObserverZ1 mot [inverted]

Microscope controls

Fully automated objective nosepiece, reflector turret and light path control

Fully motorized stage (x, y) and focus control (z)

Environmental chamber control (temperature, CO2 and humidity), on stage environmental control (temperature, CO2 and humidity).

Software

ZenPro

Capabilities

Confocal optical sectioning

Live cell imaging

Multi-channel imaging

Timelapse

Z-stack

Tile scanning

FRAP, FLIP, photo-conversion

Objectives

10x, 0.3 NA, Air, EC Plan-Neofluar (Ph1)

20x, 0.8 NA, Air, Plan-Apo

40x, 1.3 NA, Oil, Plan-Apo

63x, 1.4 NA, Oil, Plan-Apo

Light sources

X-Cite 120 mini LED and transmitted LED (visualization only- BF, Ph1, Ph2,Ph3)

4 laser lines: 405nm, 490nm, 561nm, 639nm

Filters

Variable Secondary Dichroic adjustable in 1nm increments 400nm-700nm to split light between Ch1 and Ch2

Ch1 filters
Ch 2 filters

SP 470nm

SP 620nm

SP 545nm

LP 575nm

SP 620nm

LP 655nm

Detectors

2 PMTs

Location

RGN 3140

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