Beckman MoFlo AstriosEQ

Submicron-sized particle interrogation capabilities

Beckman MoFlo AstriosEQ

Sample Loading Options

  • 12 x 75-mm polypropylene tubes
  • 1.5ml eppendorf tubes
  • 15ml tubes
  • 50ml tubes

Collection devices

  • Up to 6-way sorting into 12 x 75 mm polypropylene tubes
  • 4-way sorting: up to three 12x75mm polypropylene tubes and one 15 mL tube
  • 2-way sorting into 50ml tubes
  • One-way sorting (including single cell) onto slides and into 6, 24, 48, 96, and 384-well plates

Nozzles (nozzle should be 4-5 times the cell size)

  • 70 and 100um

Lasers & Filter Sets

Examples of fluorochromes that can be used

MoFlo AstriosEQ Configuration: 6 Lasers

405nm

405-SSC

448/59:

Pacific Blue,

BV421,V450

546/20:

BV510,

V500, QDot525

610/20:

BV605, QDot605

710/50:

BV711, QDot705

488nm

488-SSC

513/26:

FITC, eGFP

576/21:

 

664/22 or 710/45

PE-Cy5,

 PerCPCy5.5

795/70:

PE-Cy7

532nm

532-SSC

576/21:

 

622/22:

PI

664/22:

7AAD

736/47:

 

561nm

561-SSC

576/16:

PE

614/20:

mCherry

mStrawberry

692/75:

PE-Cy5.5

795/70:

PE-Cy7

592nm

620/29:

mCherry

mKate

mRaspberry

671/30:

mPlum

722/44:

 

 

640nm

671/30:

APC,

Alexa 647

722/44:

Alexa 700

795/70:

Alexa 750,

APC-Cy7

 

 

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