Submicron-sized particle interrogation capabilities
Sample Loading Options
- 12 x 75-mm polypropylene tubes
- 1.5ml eppendorf tubes
- 15ml tubes
- 50ml tubes
Collection devices
- Up to 6-way sorting into 12 x 75 mm polypropylene tubes
- 4-way sorting: up to three 12x75mm polypropylene tubes and one 15 mL tube
- 2-way sorting into 50ml tubes
- One-way sorting (including single cell) onto slides and into 6, 24, 48, 96, and 384-well plates
Nozzles (nozzle should be 4-5 times the cell size)
- 70 and 100um
Lasers & Filter Sets
Examples of fluorochromes that can be used
|
MoFlo AstriosEQ Configuration: 6 Lasers |
|||||
|
405nm |
405-SSC |
448/59: Pacific Blue, BV421,V450 |
546/20: BV510, V500, QDot525 |
610/20: BV605, QDot605 |
710/50: BV711, QDot705 |
|
488nm |
488-SSC |
513/26: FITC, eGFP |
576/21:
|
664/22 or 710/45 PE-Cy5, PerCPCy5.5 |
795/70: PE-Cy7 |
|
532nm |
532-SSC |
576/21:
|
622/22: PI |
664/22: 7AAD |
736/47:
|
|
561nm |
561-SSC |
576/16: PE |
614/20: mCherry mStrawberry |
692/75: PE-Cy5.5 |
795/70: PE-Cy7 |
|
592nm |
620/29: mCherry mKate mRaspberry |
671/30: mPlum |
722/44: |
|
|
|
640nm |
671/30: APC, Alexa 647 |
722/44: Alexa 700 |
795/70: Alexa 750, APC-Cy7 |
|
|