Immunohistochemistry

Immunohistochemistry (IHC) and immunofluorescence (IF) are specialized techniques that involve the use of antibodies to detect specific proteins (antigens) in cells of a tissue section. Proteins are visualized using either chromogenic detection with a colored enzyme substrate (IHC) or fluorescent detection with fluorescent dyes (IF).

Before submitting any samples for IHC or IF, we strongly encourage researchers to consult with us prior to antibody selection and assay design.

The Histology Core Facility offers:
  • Titration of new antibodies
  • Staining of established antibodies
  • Manual and Automated staining (Leica Bond III Immunostainer):
    • Chromogenic IHC
      • Horseradish peroxidase (HRP) in brown
      • Alkaline phosphatase (AP) in pink
      • Single or dual color
    • Immunofluorescence
      • Single or multi-color
  • In-Situ Hybridization  (Coming soon)
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