The Protein Biophysics Core Facility
The Protein Biophysics Core Facility (PBCF) is located on the 4th floor in Roger Guindon Hall, at the Faculty of Medicine, Department of Biochemistry, Microbiology and Immunology, University of Ottawa. The PBCF provides expertise, technical support, training and access to instruments for faculty members, researchers, students and external clients. The PBCF houses a wide range of instrumentations suitable for the biophysical characterization of proteins. PBCF is equipped with facilities needed for the production and purification of recombinant proteins, for protein crystallization as well as for conducting binding studies. PBCF offers services related to the sub-cloning of genes in vectors for the expression of proteins in bacteria as well as services related to the overexpression and purification of recombinant proteins in E. coli. We offer services related to the determination of equilibrium dissociation constants (KD) for a protein to its ligand using surface plasmon resonance or isothermal titration calorimetry. Finally, the core also offers services in regard to the identification of conditions yielding diffraction quality crystals and crystal structure determination of proteins.