Service packages

Single Protein Profiling

We provide multi-enzyme digestion, as well as a suite of methods, like modifying column hydrophobicity, buffer pH, CID energy etc. to help to increase sequence coverage and confidence of your target protein.
Protein ID and PTM analysis from Gel Bands

This service is done on the LTQ orbitrap XL platform for general protein identification and expected PTM or protein isoforms identifications from gel bands. It includes in-gel digestion, HPLC-ESI-MS/MS analysis (60min), Mascot search and preliminary filtration/organization of the results, as well as data storage for 2 months. To reduce the cost, customers have the option to perform the in-gel digestion themselves.
Large Scale Protein Profiling

This service aims to identify and quantify complex samples.

We employ the most reliable two dimensional strong cation exchange/reverse phase (SCX/RP) fractionation of peptides and Orbirap analysis to increase the identification of proteins. For a typical sample (e.g. total cell lysate), thousands of proteins are routinely identified with high confidence.

This service includes in-solution digestion, SCX fractionation, HPLC-ESI-MS/MS on an Orbitrap XL mass spectrometer, Mascot search results, and data storage for 2 months. Option without fractionation is available. Note that the more fractions, the more protein identified, although it's not linear.
Nonconventional Protein Analysis

We also have Velos-Pro-Orbitrap Elite, Q-Exactive, and Qtrap5500 Qtrap5500 mass spectrometers, which can be used for a large variety of analysis, like MRM quantification, single PTM, peptide/protein validation and confirmation. Please contact us for more details.

We sell established stably heavy-labeled SILAC cells for superSILAC spike-in experiments

*Cell lines*	*Organism*	*Tissue*	*Cell Type/ morphology*
ATT-20	Mouse	pituitary	round cells
CHO	Chinese hamster	ovary	epithelial-like
CLU-181	Mouse(male)	hypothalamus	neuroblast
CLU-188	Mouse(male)	hypothalamus	neuroblast
HCT-116	Human	colon	epithelial
HEK-293	Human	kidney	epithelial
HEPA 1-6	Mouse	Liver	epithelial
HuH-7	Human	liver	epithelial-like
Jurkat-E6	Human(male)	Blood	T-lymphocyte (non-adherent)
MEF-1	Mouse	embryo	fibroblast
N38	Mouse(male,embryo)	hypothalamus	neuroblast
Neuro 2A	Mouse	Brain	neuroblast
PEA 13	Mouse	embryo	fibroblast
THP-1	Human (male)	Blood	Leukocyte (non-adherent)/monocyte

Other Services (contact us for more details):

Deep bioinformatic analysis of proteomic data

Consult on design for proteomics experiments and analysis

SILAC labeled cells for quantitative spiked-in experiments

Dimethyl labeling/Multiplex labeling for protein/peptide quantitation

Small molecule (DNA/RNA drug) detection and quantitation analysis.

Long-term data storage.

Training on MS for lipidomics/proteomics

Please note:
Information

For Conventional Service:

Most of the time we do not get involved in experimental design and extra bioinformatic analysis for conventional service provided (unless stated). We keep the right of applying extra charges if extra service needed.

Service or Collaboration:

We are open to and interested in potential collaborations related to our own research projects. Please contact Professor Daniel Fiegys for potential collaborations.

Please note we charge collaboration costs, that include but are not limited to, costs for protein digestions, desalting (columns), chemicals, solvents, hydro, parts replacement and deprecations.

Service packages

Proteomics Resource Center