Services

The Behavioral Core is supported through the University of Ottawa. Users are charged an hourly fee for all use of the Facility. Such uses include experimentation and computer analysis.

The Behavioral Core charges three basic rates

• Core Staff Performed Research rate
• Direct Supervision rate
• Indirect Supervision rate

The CBIA core facility works to provide a holistic model of support to users from initial consultation, training, follow up consultations and image analysis to support your research. Basic support (excluding training) is free for users to ensure high level understanding of imaging experiments and systems used. See below for examples of support we offer to users:

• Experimental design consultation
• Fluorophore choice and microscopy platform matching
• Imaging parameter advice
• Post-acquisition support in Fiji (ImageJ) for development of analysis workflow

Training and advanced support from technical staff is a charge for use service.

Each suite has a cell culture room, procedure room and housing room for small animals. The latter has a potential capacity of 280 isolated cages (mice), and is maintained by uOttawa Animal Care and Veterinary Service. The Facility provides basic laboratory equipment while specialty equipment must be provided by individual research groups.

• User Training
• Analysis (Fortessa, Celesta)
• Cell Sorting (MoFlo Astrios)
• Analysis & Sorting for Submicron-sized Particles (Fortessa, MoFlo Astrios)
• Samples run by Trained Users
• Workstation

Welcome to the Pathology and Laboratory Medicine – Histology Core Facility

The Histology Core Facility (HCF) is located in the Faculty of Medicine, Department of Pathology and Laboratory Medicine at the University of Ottawa. It is a full-service support laboratory that provides histology services for faculty, researchers, students and external clients. The primary mission of the HCF is to provide efficient, high quality and cost-effective histological services for both animal and human tissue.

Services include:

• Paraffin processing, embedding and sectioning
• Embedding and sectioning of frozen tissue
• Routine and special stains
• Chromogenic and fluorescent immunohistochemistry staining
• Slide scanning

• Training on the use of machines (except MRI)
• Machine maintenance
• Experimental design consultation
• MRI protocol design
• MRI experiments execution, including animal handling
• MRI data supply
• MRI analysis (for some cases)

Regular Service item pricing

In-gel digestion: $80/band

In-solution digestion: $140/sampler

Desalting by SPE column: $20/sample

SCX fractionation into 5: $20/sample

MS analysis on Orbitrap XL: $60/hr

MS analysis on Orbitrap Elite or Q-Exactive: $100/hr

Database search (Xtandem or Maxquant), primary analysis and data storage for 1 year: free

• Transgenesis
• What the user will provide
• What the transgenic core facility will do
• Cryopreservation and Cryorecovery