Associate Professor, Department of Biology, Carleton University
Work: (613) 520-2600 ext. 1006
Keywords: genomics, functional genomics, proteomics, molecular biology, molecular genetics, microbial biology, anti-microbial/fungals
We are interested in identification of novel gene/protein functions in model microbial organisms such as yeast, Saccharomyces cerevisiae and E. coli. This area of biology is often called functional genomics/proteomics and/or systems molecular biology. The two main areas that we target are translation (protein synthesis) control and DNA damage repair, both of which have implications in different human conditions such as cancer and apoptosis. We focus on pure research with some implication on the applied fields such as development of novel therapeutics. We use advanced molecular biology and molecular genetics techniques for our research.
We are also interested in chemical-genomics of various natural bioactive compounds. There are 1000s of compounds found in nature, which are capable of performing various interesting functions within a cell. However their biological implications at the molecular level have not been realized until very recently. We perform systematic approaches to investigate the target site of some of these naturally occurring compounds.
We are also vigorously involved in a number of environmental engineering projects (mostly microbiology based) and bioinformatics research and computational tool developments. For example we are the first group, which computationally investigated the global map of protein-protein interactions in any organism (S. cerevisiae).
Our research interests provide an excellent opportunity for the training of undergraduate and graduate students as well as post-doctoral fellows.
Jessulat M, Buist T, Alamgir M, Hooshyar M, Xu J, Aoki H, Ganoza MC, Butland G, Golshani A. In vivo investigation of protein-protein interactions to study helicases using tandem affinity purification. Methods Mol Biol. (in press)
Sirskyj D, Weltzin R, Golshani A, Anderson D, Bozic J, Diaz-Mitoma F, Azizi A. Detection of influenza A and B neutralizing antibodies in vaccinated ferrets and macaques using specific biotin-streptavidin conjugated antibodies. J Virol Methods. (in press)
Hu P, Janga SC, Babu M, Díaz-Mejía JJ, Butland G, Yang W, Pogoutse O, Guo X, Phanse S, Wong P, Chandran S, Christopoulos C, Nazarians-Armavil A, Nasseri NK, Musso G, Ali M, Nazemof N, Eroukova V, Golshani A, Paccanaro A, Greenblatt JF, Moreno-Hagelsieb G, Emili A. Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 2009; 7(4):e96. (18 pages)
Alamgir M, Eroukova V, Jessulat M, Xu J, Golshani A. Chemical-genetic profile analysis in yeast suggests that a previously uncharacterized open reading frame, YBR261C, affects protein synthesis. BMC Genomics. 2008; 9:583. (13 pages)
Pitre S, North C, Alamgir M, Jessulat M, Chan A, Luo X, Green JR, Dumontier M, Dehne F, Golshani A. Global investigation of protein-protein interactions in yeast Saccharomyces cerevisiae using re-occurring short polypeptide sequences. Nucleic Acids Res. 2008; 36:4286-4294.
Aoki H, Xu J, Emili A, Chosay JG, Golshani A, Ganoza MC. Interactions of elongation factor EF-P with the Escherichia coli ribosome. FEBS J. 2008; 275:671-681.
Pitre S, Alamgir M, Green JR, Dumontier M, Dehne F, Golshani A. Computational methods for predicting protein-protein interactions. Adv Biochem Eng Biotechnol. 2008; 110:247-67.
Smith RP, Cruz I, Golshani A, Chesnais C, Smith ML. Secondary assays for testing the mode of action of natural products with bioactivity against fungi. Pharmaceut Biol. 2008; 46:16-25.
Jessulat M, Alamgir M, Salsali H, Greenblatt J, Xu J, Golshani A. Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae. Arch Biochem Biophys. 2008; 469:157-164.
Chepelev N, Chepelev L, Alamgir M, Golshani A. Large-scale protein-protein interaction detection approaches: past, present and future. Biotech Biotechnol Eq. 2008; 22:513-529.
Galván IJ, Mir-Rashed N, Jessulat M, Atanya M, Golshani A, Durst T, Petit P, Amiguet VT, Boekhout T, Summerbell R, Cruz I, Arnason JT, Smith ML. Antifungal and antioxidant activities of the phytomedicine pipsissewa, Chimaphila umbellata. Phytochemistry. 2008; 69:738-746.
Memarian N, Jessulat M, Alirezaie J, Mir-Rashed N, Xu J, Zareie M, Smith M, Golshani A. Colony size measurement of the yeast gene deletion strains for functional genomics. BMC Bioinformatics. 2007; 8:117 (11 pages).
Butland G, Krogan NJ, Xu J, Yang WH, Aoki H, Li JS, Krogan N, Menendez J, Cagney G, Kiani GC, Jessulat MG, Datta N, Ivanov I, Abouhaidar MG, Emili A, Greenblatt J, Ganoza MC, Golshani A. Investigating the in vivo activity of the DeaD protein using protein-protein interactions and the translational activity of structured chloramphenicol acetyltransferase mRNAs. J Cell Biochem. 2007; 100:642-652.