This facility contains a crystallization robot for the high throughput crystallization of biological macromolecules, including DNA, RNA and proteins. In addition, the core has a Rigaku MicroMAX 007-HF for structure resolution equipped with a RAXIS-4 Detector.
Our proteomic core facility has a complete series of state-of-the art mass spectrometers. We offer the research community a complete array of proteomic services from protein and PTM identification to super silac and single cell proteomics.
We provide advice on bioinformatics research design, conduct bioinformatics analysis, provide data warehousing services and provide support for grant proposals that involve bioinformatics (including conducting pilot studies, support/collaboration letters, methodological text and more).
LPR is a lipidomics and lipid-bioinformatics research platform that provides a complete range of services, from extraction, MS analysis, bioinformatics and visualization/network tools. This platform is unique in its combined application of LC-ESI-MS, bioinformatics, 3D visualization and hybrid representation softwares adapted for lipid representation, and super-resolution STED CW evaluation of membrane microdomains through our sister STEDCore suite to neurolipidomics.
STEDCore is the first super-resolution Stimulated Emission Depletion Continuous Wave (STED CW) research platform in Canada. The platform, both confocal and super-resolution systems, is operated by Mr. Mark Akins, who works with Dr. Steffany Bennett to provide researchers with imaging expertise, calibrate and maintain all of STEDCore's equipment, develop new super-resolution analysis protocols and, if requested, perform critical experiments for users.
Metabolomics Core Facility
Our Metabolomics Core Facility is part of MIRGOV, the Metabolomics Innovation Resource of the Goodman Cancer Research Centre (GCRC), the University of Ottawa and the Van Andel Research Institute (VARI). We house state-of-the-art mass spectrometers for targeted quantification of small molecules, untargeted metabolomics and stable isotope tracing analyses.
This facility contains state-of-the-art infrastructure to study protein structures, including AKTA purification systems combined with size-exclusion columns, a calorimeter for the measurement of protein-ligand thermodynamics and spectrophotomers for the study of protein secondary structures in solution. This facility also includes a crystallization robot to carry out crystallization trials with high throughput capabilities.
This facility contains a Singer ROTOR HAD, a compact benchtop robot for easy, ultra-fast manipulation of high density arrays of yeast. It also contains imaging equipment and a spectrometer for automated growth curve analysis, to allow for functional and chemical genomic yeast screening of mutant collections.