The RNAi Consortium shRNA clones for the human genome are now available

Posted on Wednesday, December 8, 2021

The RNAi Consortium (TRC) 2.0 arrayed shRNA human genome library clones are now available as plasmids in E.Coli. These plasmids can be used for generating lentivirus containing shRNA hairpin sequences that are able to knockdown the expression of specific genes in human cells.  There are about 118,000 clones covering ~20,000 genes. Individual clones can be acquired, streaked on LB amp+ plates, for $10/clone from the CHEO Research Institute HTS lab.

For more information and to see what clones are available for your genes of interest please go to our webpage http://htslab.med.uottawa.ca/trc_shrna/

Please note, this webpage is only accessible from the uOttawa (including VPN), OHRI and CHEO networks.

Vector used for generating shRNA lentivirus

Vector used for generating shRNA lentivirus

 

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